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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has actually been noticed in lots of tumors, which leads to chromosome instability, and therefore may possibly add to tumorigenesis. This protein was uncovered to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle regulated histone gene expression and plays a essential part in promoting mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode distinctive isoforms, have already been described. Two extra splice variants ended up described but detailed nucleotide sequence details isn't nonetheless offered.
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Blocking peptides are peptides that bind specially on the goal antibody and block antibody binding. These peptide generally has the epitope identified because of the antibody.
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This tutorial outlines even more achievable optimization of cellular and immunoassay parameters to ensure the very best final results are...
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This guidebook outlines additional attainable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide now not bind to your epitope over the concentrate on protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, one can see which staining is particular.
Target Information Cyclin E belongs to the really conserved cyclin spouse and children, whose customers are characterised by a dramatic periodicity in protein abundance with the cell cycle. Cyclins perform as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a complex with and functions to be a regulatory subunit of CDK2, whose action is required for mobile cycle G1/S transition. This protein accumulates with the G1-S stage boundary and is particularly degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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